LOCUS lambda_gt11 1989 bp DNA linear 19-OCT-2007 DEFINITION Cloning vector lambda_gt11, partial sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector lambda_gt11 ORGANISM Cloning vector lambda_gt11 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 1989) AUTHORS Moran,L.S., Maina,C.V., Poole,C.B. and Slatko,B.E. TITLE Nucleotide sequence of the phage lambda gt11 SacI-KpnI lacZ region JOURNAL Gene 93 (1), 163-164 (1990) PUBMED 2146187 REFERENCE 2 (bases 1 to 1989) AUTHORS Slatko,B.E. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT Sequence of the SacI-KpnI fragment surrounding the EcoRI cloning site. The remainder of lambda_gt11 has not been sequenced to date. See also GenBank accession X16973. FEATURES Location/Qualifiers source 1..1989 /organism="Cloning vector lambda_gt11" /mol_type="genomic DNA" gene <1..1125 /gene="lacZ" CDS <1..1125 /gene="lacZ" /codon_start=1 /product="beta-galactosidase" /translation="ELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIEL GNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVER WKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAIT VDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDM YTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTW LNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" primer_bind 1033..1056 /gene="lacZ" /note="lambda gt11 forward primer (NEB #S1218S; discontinued)" misc_feature 1069..1074 /gene="lacZ" /note="EcoRI cloning site" primer_bind complement(1096..1119) /gene="lacZ" /note="lambda gt11 reverse primer (NEB #S1222S; discontinued)" gene complement(1116..1580) /gene="lom" CDS complement(1116..1580) /gene="lom" /codon_start=1 /product="outer membrane protein" /translation="MRNVCIAVAVFAALAVTVTPARAEGGHGTFTVGYFQVKPGTLPS LSGGDTGVSHLKGINVKYRYELTDSVGVMASLGFAASKKSSTVMTGEDTFHYESLRGR YVSVMAGPVLQISKQVSAYAMAGVAHSRWSGSTMDFLTRNTGRHGLPGYYYF" gene complement(1642..>1989) /gene="J" CDS complement(1642..>1989) /gene="J" /codon_start=1 /product="tail:host specificity protein" /translation="GTRTVTVTDDHPFDRQIVVLPLTFRGSKRTVSGRTTYSMCYLKV LMNGAVIYDGAANEAVQVFSRIVDMPAGRGNVILTFTLTSTRHSADIPPYTFASDVQV MVIKKQALGISVV" BASE COUNT 471 a 597 c 518 g 403 t ORIGIN 1 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct 61 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag 121 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca 181 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg 241 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc 301 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg 361 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg 421 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc 481 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca 541 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta 601 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat 661 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg 721 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc 781 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac 841 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac 901 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa 961 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc 1021 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg 1081 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg 1141 ccatgtctgc ccgtatttcg cgtaaggaaa tccattgtac tgccggacca ccgactgtga 1201 gccactccgg ccatggcgta cgcactgacc tgcttactga tttgtaaaac cggtccggcc 1261 atcacgctca cataacgtcc acgcaggctc tcatagtgaa acgtatcctc cccggtcatc 1321 actgtgctgc tctttttcga cgcggcgaac cccagggaag ccatcacccc cacactgtcc 1381 gtcagctcat aacggtactt cacgttaatc cctttcagat gactcacacc ggtatccccg 1441 cccgacaacg acggcaatgt acccggtttc acttgaaaat agcccaccgt aaacgtacca 1501 tgtccacctt ccgcacgggc cggagtgact gtcaccgcaa gtgcggcaaa gacagcaacg 1561 gcaatacaca cattacgcat cgttcacctc tcactgtttt ataataaaac gcccgttccc 1621 ggacgaacct ctgtaacaca ctcagaccac gctgatgccc agcgcctgtt tcttaatcac 1681 cataacctgc acatcgctgg caaacgtata cggcggaata tctgccgaat gccgtgtgga 1741 cgtaagcgtg aacgtcagga tcacgtttcc ccgacccgct ggcatgtcaa caatacggga 1801 gaacacctgt accgcctcgt tcgccgcgcc atcataaatc accgcaccgt tcatcagtac 1861 tttcagataa cacatcgaat acgttgtcct gccgctgaca gtacgcttac ttccgcgaaa 1921 cgtcagcgga agcaccacta tctggcgatc aaaaggatgg tcatcggtca cggtgacagt 1981 acgggtacc //