LOCUS pGT-N28 4665 bp DNA circular 05-DEC-2007 DEFINITION Cloning vector pGT-N28, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pGT-N28 ORGANISM Cloning vector pGT-N28 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4665) AUTHORS Polakiewicz,R.D. TITLE Direct Submission JOURNAL Submitted (05-DEC-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..4665 /organism="Cloning vector pGT-N28" /mol_type="other DNA" gene 143..1003 /gene="bla" CDS 143..1003 /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide 143..211 /gene="bla" /note="Required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." rep_origin complement(1045..1554) /note="M13 origin of replication (+ -)" rep_origin 1665..2253 /note="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" misc_feature 2465..2544 /note="multiple cloning site I (NotI-ApaI)" promoter 2535..3072 /note="phosphoglycerate kinase (PGK) promoter region" gene 3073..3876 /gene="aph(3')-II" CDS 3073..3876 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD LDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3877..4364 /note="PGK polyA" misc_feature 4360..4450 /note="multiple cloning site II (XbaI-AflII)" BASE COUNT 1102 a 1183 c 1240 g 1140 t ORIGIN 1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct 361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg 1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta 1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca 1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta 1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga 1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg 1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc 2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca 2461 ctaggcggcc gcctagtggg cagatcttcg aatgcatcgc gcgcaccgta cgtctcgagg 2521 aattcctgca ggattcgagg gcccctgcag gtcaattcta ccgggtaggg gaggcgcttt 2581 tcccaaggca gtctggagca tgcgctttag cagccccgct ggcacttggc gctacacaag 2641 tggcctctgg cctcgcacac attccacatc caccggtagc gccaaccggc tccgttcttt 2701 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg 2761 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag 2821 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag 2881 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc 2941 aggggcgggc tcaggggcgg ggcgggcgcg aaggtcctcc cgaggcccgg cattctcgca 3001 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga 3061 cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc 3121 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 3181 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3241 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 3301 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 3361 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 3421 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 3481 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 3541 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 3601 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg 3661 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 3721 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 3781 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 3841 atcgccttct atcgccttct tgacgagttc ttctgagggg atcgatccgt cctgtaagtc 3901 tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg 3961 tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag 4021 ctacgggggt gggggtgggg tgggattaga taaatgcctg ctctttactg aaggctcttt 4081 actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa 4141 attaagggcc agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga 4201 tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt 4261 cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca 4321 gtattgtttt gccaagttct aattccatca gaagctgact ctagatctgg atccacgaag 4381 cttcccatgg tgacgtcacc ggttctagat acctaggtga gctctggtac cctctagtca 4441 aggccttaag tgagtcgtat tacggactgg ccgtcgtttt acaacgtcgt gactgggaaa 4501 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 4561 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 4621 ggcgcttcgc ttggtaataa agcccgcttc ggcgggcttt ttttt //