LOCUS pGT-N29 4662 bp DNA circular 07-DEC-2007 DEFINITION Cloning vector pGT-N29, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pGT-N29 ORGANISM Cloning vector pGT-N29 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4662) AUTHORS Polakiewicz,R.D. TITLE Direct Submission JOURNAL Submitted (07-DEC-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..4662 /organism="Cloning vector pGT-N29" /mol_type="other DNA" gene 143..1003 /gene="bla" CDS 143..1003 /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide 143..211 /gene="bla" /note="Required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." rep_origin complement(1045..1554) /note="M13 origin of replication (+ -)" rep_origin 1665..2253 /note="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" misc_feature 2465..2553 /note="multiple cloning site I (NotI-ApaI)" promoter 2544..3081 /note="phosphoglycerate kinase (PGK) promoter region" gene 3082..3885 /gene="aph(3')-II" CDS 3082..3885 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD LDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3886..4373 /note="PGK polyA" misc_feature 4369..4447 /note="multiple cloning site II (XbaI-AflII)" BASE COUNT 1102 a 1184 c 1238 g 1138 t ORIGIN 1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct 361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg 1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta 1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca 1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta 1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga 1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg 1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc 2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca 2461 ctaggcggcc gcctagtggg taccagagct ccctaggttc tagaaccggt gacgtctccc 2521 atggtgaagc ttggatccac gattcgaggg cccctgcagg tcaattctac cgggtagggg 2581 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg gcacttggcg 2641 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtagcg ccaaccggct 2701 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc 2761 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt 2821 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc 2881 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg 2941 ggcgggctca ggggcgggct caggggcggg gcgggcgcga aggtcctccc gaggcccggc 3001 attctcgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg 3061 gcctttcgac ctgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt 3121 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 3181 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 3241 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg 3301 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 3361 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 3421 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 3481 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 3541 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 3601 ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat 3661 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 3721 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 3781 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 3841 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagggga tcgatccgtc 3901 ctgtaagtct gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag 3961 tttttcctgt catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa 4021 ggattggagc tacgggggtg ggggtggggt gggattagat aaatgcctgc tctttactga 4081 aggctcttta ctattgcttt atgataatgt ttcatagttg gatatcataa tttaaacaag 4141 caaaaccaaa ttaagggcca gctcattcct cccactcatg atctatagat ctatagatct 4201 ctcgtgggat cattgttttt ctcttgattc ccactttgtg gttctaagta ctgtggtttc 4261 caaatgtgtc agtttcatag cctgaagaac gagatcagca gcctctgttc cacatacact 4321 tcattctcag tattgttttg ccaagttcta attccatcag aagctgactc tagatcctgc 4381 aggaattcct cgagaccgta cgtgcgcgcg aatgcatcca gatcttccct ctagtcaagg 4441 ccttaagtga gtcgtattac ggactggccg tcgttttaca acgtcgtgac tgggaaaacc 4501 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 4561 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc 4621 gcttcgcttg gtaataaagc ccgcttcggc gggctttttt tt //