LOCUS pMAL-c6T 5247 bp DNA circular UNA 10-MAR-2020 DEFINITION . ACCESSION urn.local...kc-bba4i38 VERSION urn.local...kc-bba4i38 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers -35_signal 14..18 gene 14..1162 /gene="lacIq" /standard_name="lacIq gene" -10_signal 39..45 CDS 80..1162 /gene="lacIq" /codon_start=1 /transl_table=11 /product="lac repressor (LacI)" /standard_name="lacIq CDS" -35_signal 1405..1410 gene 1405..2982 /standard_name="malE expression cassette gene" -10_signal 1427..1432 CDS 1527..2768 /standard_name="His-malE expression cassette CDS" /note="TEV site 2694..2711." misc_feature 1536..1553 /standard_name="6x polyhistidine tag" primer_bind 2604..2627 /note="malE primer sequence" /standard_name="malE primer sequence primer bind" misc_feature 2722..2761 /standard_name="Multiple cloning site" terminator 2780..2823 primer_bind complement(2837..2860) /note="MCS reverse primer sequence" /standard_name="MCS reverse primer sequence primer bind" terminator 2955..2982 -35_signal 3031..3036 gene 3031..3961 /gene="bla" /standard_name="bla gene" -10_signal 3054..3059 CDS 3101..3961 /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /standard_name="bla CDS" rep_origin 4049..4637 CDS complement(5007..5198) /gene="rop" /codon_start=1 /transl_table=11 /product="ROP protein" /standard_name="rop CDS" gene complement(5007..5198) /gene="rop" /standard_name="rop gene" ORIGIN 1 cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag 61 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg 121 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac 181 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca 241 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca 301 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt 361 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct 421 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat 481 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac 541 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct 601 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc 661 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc 721 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa 781 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat 841 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atttcggtag tgggatacga 901 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg 961 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa 1021 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac 1081 acaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 1141 ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg 1201 cacaattctc atgtttgaca gcttatcatc gactgcacgg tgcaccaatg cttctggcgt 1261 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt 1321 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc 1381 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa 1441 ttgtgagcgg ataacaattt cacacaggaa acagccagtc cgtttaggtg ttttcacgag 1501 caattgacca acaaggacca tagattatga aaatccacca tcaccaccac cacgaagaag 1561 gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta 1621 agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag 1681 agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg 1741 accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt 1801 tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg 1861 cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc 1921 cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga 1981 gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg 2041 ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata 2101 acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga 2161 atgcagacac cgattactcc atcgcagaag cagcctttaa taaaggcgaa acagcgatga 2221 ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa 2281 cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag 2341 gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc 2401 tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga 2461 agtcttacga ggaagagttg gtgaaagatc cgcgtattgc cgccactatg gaaaacgccc 2521 agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta 2581 ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc 2641 agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc ggggagaacc 2701 tgtacttcca gatgctgatg ggcggccgcg atatcgtcga cggatccgaa ttccctgcag 2761 gtaattaaat aagcttcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2821 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt 2881 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag 2941 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct 3001 ttcggtccgt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 3061 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 3121 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 3181 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 3241 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt tcccaatgat 3301 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga 3361 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 3421 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 3481 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 3541 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 3601 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 3661 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 3721 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 3781 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 3841 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 3901 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 3961 actgtcagac caagtttact catatatact ttagattgat ttccttagga ctgagcgtca 4021 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 4081 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 4141 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 4201 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 4261 ctctgctaat cctgttacca gtgggtgctg ccagtggcga taagtcgtgt cttaccgggt 4321 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 4381 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 4441 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 4501 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 4561 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 4621 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 4681 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 4741 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 4801 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 4861 tttcacaccg catataaggt gcactgtgac tgggtcatgg ctgcgccccg acacccgcca 4921 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 4981 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 5041 aggcagctgc ggtaaagctc atcagcgtgg tcgtgcagcg attcacagat gtctgcctgt 5101 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag 5161 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaagctcag 5221 gccaggaccc aacgctgccc gaaattc //