LOCUS pMiniT 2525 bp DNA circular 11-APR-2017 DEFINITION Cloning vector pMiniT, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pMiniT ORGANISM Cloning vector pMiniT other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2525) AUTHORS Kucera,R.B. TITLE Direct Submission JOURNAL Submitted (11-APR-2017) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..2525 /organism="Cloning vector pMiniT" /mol_type="other DNA" regulatory 1..214 /regulatory_class="promoter" /note="constitutive promoter region" primer_bind 391..412 /note="cloning analysis forward primer sequence" gene 518..526 /gene="toxic minigene" CDS 518..526 /gene="toxic minigene" /note="2 additional stop codons beyond gene boundary" /codon_start=1 /product="Met-Ile" /translation="MI" misc_feature 522..523 /gene="toxic minigene" /note="blunt end cleavage site; product is supplied in linearized form, cut between these two bases" primer_bind complement(614..636) /note="cloning analysis reverse primer sequence" gene 670..1530 /gene="bla" CDS 670..1530 /gene="bla" /note="ampR (confers resistance to ampicillin) (mutant V82I,A182V)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 1701..2289 /note="pUC19 origin of replication (RNAII -35 to RNA/DNA switch point)" BASE COUNT 685 a 608 c 602 g 630 t ORIGIN 1 ccggttcaga caggataaag aggaacgcag aatgttagac aacacccgct tacgcatagc 61 tattcagaaa tcaggccgtt taagcgatga ttcacgagaa ttgctggccc gctgcggcat 121 aaaaattaat ttacacactc agcgctgatg aatcccctaa tgattttggt aaaaatcatt 181 aagttaaggt ggacacacat cttgtcatat gattaaatgg tttcgcgaaa aatcaataat 241 cagacaacaa gatgtgcgaa ctcgatattt tacacgactc tctttaccaa ttctgccccg 301 aattacactt aaaacgactc aacagcttaa cgttggcttg ccacgcatta cttgactgta 361 aaactctcac tcttaccgaa cttggccgta acctgccaac caaagcgaga acaaaacata 421 acatcaaacg aatcgaccga ttgttaggta atcgtcacct ccacaaagag cgactcgctg 481 tatcgctcga gggatccgaa ttcaggaggt aaaaaccatg atctgataat aatgacgtca 541 gaattctcga gtcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 601 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 661 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 721 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 781 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 841 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 901 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 961 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 1021 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 1081 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 1141 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 1201 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 1261 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 1321 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 1381 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 1441 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 1501 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 1561 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 1621 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 1681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 1741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 1801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 1861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 1921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 1981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 2041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 2101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 2161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 2221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 2281 ctatggaaaa acgccagcaa tgcggccttt ttacggttcc tggccttttg ctggcctttt 2341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 2401 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 2461 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 2521 tgcag //