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Product Information
BbsI has a High Fidelity version BbsI-HF® (NEB #R3539).
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.
Product Source
An E. coli strain that carries the cloned BbsI gene from Bacillus brevis.
- This product is related to the following categories:
- Restriction Endonucleases B Products,
- Time-Saver Qualified Restriction Enzymes Products
- This product can be used in the following applications:
- NEBridge® Golden Gate Assembly ,
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
- Restriction Enzyme Digestion
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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- Restriction Enzymes in Golden Gate Assembly
- Restriction Enzymes in Golden Gate Assembly
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Citations & Technical Literature
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Product Citation Tool
Featured Videos
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Golden Gate Assembly Workflow
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Golden Gate Assembly Domestication Tutorial
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Listen to DAD (Data-optimized Assembly Design) when constructing high-complexity Golden Gate Assembly targets
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Golden Gate Assembly Tool Tutorial
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Restriction Enzymes in Golden Gate Assembly
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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TIME-SAVER™ Protocol for Restriction Enzyme Digests
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NEB® TV Ep. 15 – Applications of Restriction Enzymes
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Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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Why is My Restriction Enzyme Not Cutting DNA?
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Restriction Enzyme Digest Problem: Too Many DNA Bands
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Double Digestion with NEBcloner
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