Immunoprecipitation (IP) is a method for selectively isolating a particular protein species from a complex protein solution. The technique relies on antigen/antibody recognition (immuno) and the subsequent mass- or affinity-related isolation of the capture/target complex (precipitation). It is the precipitation of the target protein that is referenced by the name: Pull-down assay. Pull-down assays are commonly used to either confirm the presence of a scant protein species or enrich population for a particular protein, as well as to identify natural binding partners for the captured protein. A typical pull-down assay involves the use of a matrix-associated antibody, such as a bead, and various wash and elution steps to ensure the specific preference for the targeted protein of interest.
- Cross-linking of IgG to Protein A or G Beads
- Goat Anti-Mouse IgG Magnetic Beads Protocol
- Immunoprecipitation using Protein A/G Magnetic Beads
- Isolation of MBP-fusion protein using Amylose Magnetic Beads
- Phage Display: Solution-phase Panning with Affinity Bead Capture
- Purification of IgG using Protein A/G Magnetic Beads
- Use SNAP-Capture Magnetic Beads (S9145)
- Use SNAP-Capture Pull Down Resin (S9144)
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