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RNA Analysis

Historically the function of RNA in the cell was to assist in the translation of genetic information from DNA into protein. The major species of RNA were; (A) messenger RNA, which converts the genetic information of DNA into RNA, (B) transfer RNA (tRNA) that are charged with specific amino acids and, (C) ribosomal RNA (rRNA), a major component of the ribosome.  RNA has now been implicated in a diverse number of biological processes including catalysis and transcriptional regulation. Recently, technological advances and improvements in RNA analysis and detection have led to the discovery of many new classes of small and large non-coding RNAs with novel regulatory functions.  Examples include, microRNA (miRNA), circular RNA, long  non-coding RNA (lncRNA), small nucleolar RNA (snoRNA) and extracellular RNA (exRNA). In addition, RNA modifications have revealed added complexity to RNA. These biologically relevant modifications are an active area of exploration. These findings have helped usher in a renaissance of RNA-focused research in biology.

RNA Analysis

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RNA Analysis includes these areas of focus:
Cappable-seq
In vitro Synthesis (IVT)
RNA Labeling
RNA Modification
RNA Purification and Isolation
RNA-seq
RT-qPCR, RT-PCR and cDNA Synthesis
Small RNA Detection and Isolation
    Publications related to RNA Analysis
  1. Wu, M.Z., Asahara, H., Tzertzinis, G., Roy, B. 2020. Synthesis of low immunogenicity RNA with high-temperature in vitro transcription . , PubMedID: 31900329, DOI:
  2. Wulf, Madalee; Buswell, John; Chan, Siuhong; Dai, Nan; Marks, Katherine; Tzertzinis, George; Whipple, Joe; Correa, Ivan; Schildkraut, Ira; 2019. The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping Scientific Reports. 9 (1), PubMedID: , DOI: 10.1038/s41598-019-45083-5
  3. Eyler, D.E., Franco, M.K., Batool, Z., Wu, M.Z., Dubuke, M.L., Dobosz-Bartoszek, M., Jones, J.D., Polikanov, Y.S., Roy, B., Koutmou, K.S 2019. Pseudouridinylatio of mRNA coding sequences alters translation Proc Natl Acad Sci USA. 116(46), PubMedID: 31672910, DOI: 10.1073/pnas.1821754116
  4. Wulf, M.G., Maguire, S., Humbert, P., Dai, N., Bei, Y., Nichols, N.M., Correa, I.R., Jr., Guan, S 2019. Non templated addition and template switching by Moloney murine leukemia virus MMLV based reverse transcriptases co occur and compete with each other J. Biol. Chem. 294(48), PubMedID: 31640989, DOI: 10.1074/jbc.RA119.010676
  5. Potapov, V., Fu, X., Dai, N., Correa, I.R., Jr., Tanner, N.A., Ong, J.L 2018. Base modificatons affecting RNA polymerase and reverse transcriptase fidelity Nucleic Acids Research. 46(11): 5753-5763, PubMedID: 29750267, DOI:
  6. Fuchs, R.T., Sun, Z., Zhuang, F., et al. 2015. Bias in ligation-based small RNA sequencing library construction is determined by adaptor and RNA structure PLoS One. 10(5), PubMedID: 25942392, DOI: 10.1371/journal.pone.0126049
Recommended HiScribe RNA Synthesis Kits by Application
mRNA Decapping Selection Chart
RNA Polymerase Selection Chart
NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.
RNA Ligase Selection Chart
NEB offers a variety of ligases for RNA research with unique specificities.
cDNA Synthesis Selection Chart
NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.
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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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