version 1.0
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NEBridge SplitSet
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Tool
Submit a new job
Assembly name
Assembly type
Linear
Circular
Overhang length
4-base
3-base
Ligation conditions
T4 DNA Ligase, 25°C, 1h incubation (4-base substrate)
T4 DNA Ligase, 25°C, 18h incubation (4-base substrate)
T4 DNA Ligase, 37°C, 1h incubation (4-base substrate)
T4 DNA Ligase, 37°C, 18h incubation (4-base substrate)
T7 DNA Ligase, 25°C, 18h incubation (4-base substrate)
T7 DNA Ligase, 37°C, 18h incubation (4-base substrate)
BsaI-HFv2 37-16 cycling
BsaI-HFv2 37 static
BsmBI-v2 42-16 cycling
Esp3I, 1x T4 DNA Ligase buffer, 37-16 cycling
BsaI-HFv2, 1x NEBridge Ligase MM, 37-16 cycling
PaqCI, 1x T4 DNA Ligase buffer, 37-16 cycling
PaqCI, 1x NEBridge Ligase MM, 37-16 cycling
BsmBI-v2, 1x NEBridge Ligase MM, 42-16 cycling
BbsI-HF, 1x NEBridge Ligase MM, 37-16 cycling
Nucleotide sequence
Use terminal overhangs as fusion sites (for linear assemblies only)
Define split regions based on
Number of fragments
Maximal fragment size
Minimal fragment size
Split regions
Retrieve an existing job
Request ID
HELP
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NEBridge SplitSet® Tool