Home FAQs I currently use MessageMAX™ T7 ARCA-capped Message Transcription Kit, which mRNA synthesis kit from NEB should I use?

FAQ: I currently use MessageMAX™ T7 ARCA-capped Message Transcription Kit, which mRNA synthesis kit from NEB should I use?

We recommend the HiScribe T7 ARCA mRNA kit (E2065) which will co-transcriptionally cap with ARCA but does not include Poly(A) polymerase. For use when enzymatic Poly(A) tailing is not needed, or the Poly(A) tail is encoded in the template. The HiScribe kit contains reagents and protocols for co-transcriptional ARCA synthesis and capping of 20 synthesis reactions.