FAQ: Do detergents inhibit Endo F2 activity?

Endo F2 is typically used under non-denaturing (native) conditions, as denaturation is not necessary for optimal activity of the enzyme. However, Endo F2 is active in denaturing conditions. Concentrations of 0.5% SDS do not inhibit Endo F2 activity when used in the presence of 1% NP-40.

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Endo F2