The purity of the DNA template is very important and should be prepared by purification methods such as spin column or phenol:chloroform extraction. In addition, to avoid RNase contamination we recommend wearing gloves and using filter tips and RNase-free tubes.
It is important to mix the reaction components well after thawing and to set the reaction up at room temperature in the order listed in the protocol for optimal yield.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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