FAQ: What is the maximum binding capacity of the Monarch RNA Cleanup Column provided with the Monarch RNA Cleanup Kit?

Although in some cases greater yields have been observed, the matrix supplied in each column is sufficient to bind the following quantities of RNA:

T2030/T2037: 10 µg of RNA

T2040/T2047: 50 µg of RNA

T2050/T2057: 500 µg of RNA

It is important to note that enzymatic reactions introduce components such as detergents, dyes, protein and nucleotides, all of which can compete for binding to the membrane during cleanup and affect RNA recovery.