Home FAQs Numerous publications describe the addition of various additives (e.g., betaine and PEG) to improve template switching. Can I add any of those to my reaction?

FAQ: Numerous publications describe the addition of various additives (e.g., betaine and PEG) to improve template switching. Can I add any of those to my reaction?

The Template Switching RT Enzyme Mix and Buffer have been extensively optimized for template switching. Because this development has already been done, we generally observe the best performance in the reagents as provided.