FAQ: What type of template switching oligos (TSOs) are compatible with the Template Switching RT Enzyme Mix?
 

In general, different TSOs can be used including TSOs with 5′ modifications, such as biotin, isomeric bases or abasic spacers. We recommend a TSO with rGrGrG at the 3′ end for efficient template switching but various types/sequences of TSOs are compatible with the Template Switching RT Enzyme Mix. Note that for downstream amplification using archaeal family B-type polymerases (e.g. Q5® and Phusion®), a TSO containing rU residues may cause inhibition.

For a specific application, please refer to General Guideline/TSO in the respective protocol.