If trying to ligate cohesive ends, we recommend using Hi-T4 DNA Ligase in 1X T4 DNA Ligase Buffer.
If trying to ligate blunt ends or single-base overhangs, Hi-T4 DNA Ligase should be used in our PEG-containing StickTogether™ DNA Ligase Buffer. Do not heat kill the reaction, as heat treating the PEG in the StickTogether™ DNA Ligase Buffer will inhibit transformation.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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