NEBinspired Blog https://www.neb.com/en-us/nebinspired-blog/feed NEBinspired Blog Mon, 25 Mar 2024 00:00:00 Z https://www.neb.com/en-us/nebinspired-blog 0df02451-66ec-4451-ab3e-5d2e9ea54657 https://www.neb.com/en-us/nebinspired-blog/navigating-mrna-ivt Navigating mRNA IVT <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/navigating-mrna-ivt/mrna_ivt_blog_thumb_0324.jpg?rev=8c7f8405f751492ab9ace0c782d30e19&sc_lang=en-us'/><p>Strategies for mRNA IVT templates, synthesis, capping and tailing, and purification that can streamline scale up or scale out for regulated markets.</p> Mon, 25 Mar 2024 00:00:00 Z 2024-03-25T21:47:56Z cd8f5265-e4b3-41d0-baab-ee834c0f406e https://www.neb.com/en-us/nebinspired-blog/e5hmc-seq-is-a-sequencing-method-for-direct-5hmc-detection-that-leverages-the-improved-data-quality-from-enzymatic-conversion E5hmC-seq&trade;&nbsp;<sup></sup>is a Sequencing Method for Direct 5hmC Detection that Leverages Improved Data Quality from Enzymatic Conversion <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/e5hmcseq-is-a-direct-sequencing-method/e5hmcseq_blog_thumb_0224-(1).jpg?rev=0a11f6a7a35d442aa938bedf07d3e64f&sc_lang=en-us'/><p>Learn about a new method to directly detect 5hmC at single-base resolution in this interview with NEB scientist, Dan Evanich, Ph.D..</p> Fri, 01 Mar 2024 00:00:00 Z 2024-03-05T14:35:58Z 4994ab1a-6943-40e2-b0f1-7646b2afc43b https://www.neb.com/en-us/nebinspired-blog/four-alternative-and-powerful-cloning-applications-of-nebuilder-hifi-dna-assembly Four alternative and powerful cloning applications of NEBuilder<sup>&reg;</sup> HiFi DNA assembly <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/four-alternative-and-powerful-cloning-applications-of-nebuilder-hifi-dna-assembly/nebuilderinfo_blog_thumb_0124.jpg?rev=c8aef1d2041342049e7be96a825ceb39&sc_lang=en-us'/><p>Explore the versatility of NEBuilder HiFi DNA Assembly in our latest blog. Expand your toolkit with four alternative applications for DNA manipulation.</p> Mon, 08 Jan 2024 00:00:00 Z 2024-02-02T15:22:29Z 2334e539-2107-49f6-8b9a-8bef49521fb1 https://www.neb.com/en-us/nebinspired-blog/the-vital-role-of-restriction-enzymes-in-molecular-cloning-and-dna-assembly The Vital Role of Restriction Enzymes in Molecular Cloning and DNA Assembly&nbsp; <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/the-vital-role-of-restriction-enzymes-in-molecular-cloning-and-dna-assembly/restrictionenzyme101_blog_thumb_1123.jpg?rev=3860ba95a8674f399f67764d688888a5&sc_lang=en-us'/><p>The functionality of restriction enzymes extends beyond bacterial defense mechanisms, with pivotal applications in genetic engineering processes.</p> Fri, 08 Dec 2023 00:00:00 Z 2023-12-11T18:35:18Z 28debda4-d7d7-41bc-8f3c-ad212918ab1d https://www.neb.com/en-us/nebinspired-blog/overcoming-challenges-in-ffpe-dna-library-prep Overcoming challenges in FFPE DNA library prep <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/overcoming-challenges-in-ffpe-dna-library-prep/ffpe_blog_thumb_1023.jpg?rev=b759c4606ce54c8faa3b8b5fe30a497c&sc_lang=en-us'/><p>Formalin-fixed paraffin-embedded (FFPE) samples hold invaluable genetic insights, yet library preparation is notoriously challenging. FFPE preservation processes damage DNA, resulting in low yields, non-uniform ends, and other issues. Our advanced enzymatic solutions tackle these challenges, enabling the full utilization of FFPE samples for genetic analysis.</p> Wed, 25 Oct 2023 00:00:00 Z 2023-11-02T17:46:07Z 2cdae525-25ee-4aaa-b2c1-4ae7e9a5141f https://www.neb.com/en-us/nebinspired-blog/superior-error-detection-using-authenticase-enhances-assembled-gene-synthesis Superior error detection using Authenticase<sup>&reg;</sup> enhances assembled gene synthesis <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/error-correction-and-authenticase/errorcorrection_blog_thumb_0923.jpg?rev=44062677e0df48b4932c361dc2b027a8&sc_lang=en-us'/><p>Gene synthesis often grapples with residual errors in DNA fragments, making traditional error detection methods labor-intensive and expensive. Authenticase&trade; employs structure-specific enzymes for proficient error correction. Paired with Q5&reg; High-Fidelity DNA Polymerase, it enhances DNA quality and simplifies the synthesis workflow.</p> Tue, 05 Sep 2023 00:00:00 Z 2024-02-16T01:46:27Z ee93edbb-1d3d-49e0-b785-a79f1a480d5d https://www.neb.com/en-us/nebinspired-blog/sequencing-technologies-and-bioinformatics Sequencing Technologies and Bioinformatics: From data to workflow improvements&nbsp; <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/sequencing-technologies-and-bioinformatics/bioinformatics_blog_thumb_0723.jpg?rev=81c7dbac010f4994a557f8e5a5406763&sc_lang=en-us'/><p>Bioinformatics and computational biology relate to the analysis, interpretation and application of the data collected through biological research.&nbsp;In this blog post, we explore bioinformatics applications to genetic sequencing and the ways proximity between molecular biologists and bioinformatic scientists accelerates progress, amplifying research and product development impacts.</p> Fri, 04 Aug 2023 00:00:00 Z 2023-08-04T14:56:10Z 2e55750b-c54d-4092-b00d-0dbb086497cb https://www.neb.com/en-us/nebinspired-blog/mutate-with-confidence-tips-and-tools-for-successful-site-directed-mutagenesis-experiments Mutate with Confidence! Tips and tools for successful site-directed mutagenesis experiments <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/mutate-with-confidence-tips-and-tools-for-successful-site-directed-mutagenesis-experiments/cloningmutagenesis_blog_thumb_0623.jpg?rev=11222e49d0b34dd284acae98c5a1e086&sc_lang=en-us'/><p>Site-directed mutagenesis methodologies have evolved from complex and time-consuming multi-day workflows to more efficient and streamlined protocols. In this blog post, we have compiled tips, tools and valuable insights from our scientists to maximize your efficiency and ensure your success.</p> Mon, 26 Jun 2023 00:00:00 Z 2023-06-28T18:12:47Z 635ed542-a85f-4d52-a07a-886cabcb550d https://www.neb.com/en-us/nebinspired-blog/adding-capabilities-to-crispr-cas-based-biotechnology Adding Capabilities to CRISPR-Cas-Based Biotechnology <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/adding-capabilities-to-crispr/crispr_tools_blog_thumb_0523.jpg?rev=a25ebb671e26424d85687529d84fd35d&sc_lang=en-us'/><p>The Robb Lab at NEB collaborates with both academic and industry researchers in their mission of discovery, characterization, and application development efforts with CRISPR-Cas systems. It&rsquo;s always galvanizing to look to the origin of CRISPR tools and consider how this scientific field has expanded into multiple substrate types and applications over time. While NEB produces many useful Cas nucleases to support research and development, the Robb Lab is always pursuing novel, distinctive properties &ndash; inspired by the abundance and diversity of Cas nucleases found in nature.</p> Tue, 30 May 2023 00:00:00 Z 2023-08-16T13:25:33Z c9ecbf8f-706a-4a43-b545-3feee281ac0b https://www.neb.com/en-us/nebinspired-blog/genes-in-space-is-a-unique-opportunity-for-young-scientists-to-launch-their-ideas-into-orbit Genes in Space&trade; is a unique opportunity for young scientists to launch their ideas into orbit <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/genes-in-space-is-a-unique-opportunity-for-young-scientists-to-launch-their-ideas-into-orbit/nebinspired_genesinspace_blog_1200x1200_0523.jpg?rev=de51966b5b6a403ab8623ad2eec6030f&sc_lang=en-us'/><p>Learn how Pristine Onuoha's curiosity led to a space-ready assay that could detect differences in DNA fragment length, and the implications this has for bio-monitoring DNA changes in astronauts. Her experiment is set to launch to the International Space Station in June 2023.</p> Thu, 04 May 2023 00:00:00 Z 2023-05-03T01:20:21Z 51e25982-c8c7-4377-af61-dabd13e5a7d3 https://www.neb.com/en-us/nebinspired-blog/composting---an-unsung-hero-in-sustainability Composting - an unsung hero in sustainability <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/composting-an-unsung-hero-of-sustainability/composting_blog_thumb_0423.jpg?rev=e894e9a9fbd14e94905bd384754872c3&sc_lang=en-us'/><p>Many of us are aware of the challenges with plastic waste, and the crisis facing our oceans today. However, we don&rsquo;t often think about organic waste and its environmental impacts. Luckily, the practice of composting can help manage organic waste and improve the sustainability of gardens and farms.</p> Thu, 13 Apr 2023 00:00:00 Z 2023-04-14T13:58:53Z 75896f0a-f4ab-4cc2-af9b-bc2963948ace https://www.neb.com/en-us/nebinspired-blog/nurturing-artistic-talent-and-reviving-forgotten-narratives-indigo-arts-alliance Nurturing artistic talent and reviving forgotten narratives: Indigo Arts Alliance's mission <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/nurturing-artistic-talent-and-reviving-forgotten-narratives-indigo-arts-alliances-mission/indigoartsalliance_blog_thumb_0223.jpg?rev=6cac44b7c9054c27b3e858afbd35bf49&sc_lang=en-us'/><p>In our latest blog post, learn about how Indigo Arts Alliance have created a space that fosters connections between underrepresented Black and Brown artists, and how their collaborations embrace the shared history and experiences of the African diaspora.</p> Thu, 23 Mar 2023 00:00:00 Z 2023-03-28T17:28:28Z 65142e79-5b5b-4b78-b47d-4d0856b5fff1 https://www.neb.com/en-us/nebinspired-blog/debunking-the-myths---clearing-up-six-common-misconceptions-about-reagent-lyophilization Debunking the myths: clearing up six common misconceptions about reagent lyophilization <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/debunking-the-myths---clearing-up-six-common-misconceptions-about-reagent-lyophilization/lyophilization_blog_thumb_0323.jpg?rev=886c52fc2993469390bd010b359f0b11&sc_lang=en-us'/><p>Some misconceptions about lyophilization may prevent researchers from taking full advantage of its benefits. New England Biolabs Lyophilization Sciences&trade; Ltd. has extensive experience lyophilizing reagents for life science research and applied and diagnostics projects. In this blog post, you'll find answers to some of the most common questions our scientists are asked.</p> Fri, 03 Mar 2023 00:00:00 Z 2023-03-08T19:16:44Z 921b82c8-3329-41d4-b821-66469a6a6643 https://www.neb.com/en-us/nebinspired-blog/support-for-educators-provides-an-opportunity-to-teach-hands-on-molecular-biology-techniques Support for educators provides an opportunity to teach hands-on molecular biology techniques <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/support-for-educators-provides-an-opportunity-to-teach-hands-on-molecular-biology-techniques/dei_stem_donation_blog_thumb_1222.jpg?rev=a66c38aed937475f8555ead563bb9bd3&sc_lang=en-us'/><p>The New England Biolabs<sup>&reg;</sup> DE&amp;I Committee granted lab equipment to two passionate science teachers to enhance learning experiences in molecular biology.</p> Thu, 08 Dec 2022 00:00:00 Z 2022-12-12T19:47:38Z 99a400f0-15e5-4fb4-a78f-c265ac9c3387 https://www.neb.com/en-us/nebinspired-blog/what-is-the-difference-between-intron-encoded-and-retroviral-reverse-transcriptases What is the difference between intron-encoded and retroviral reverse transcriptases? <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/what-is-the-difference-between-intron-encoded-and-retroviral-reverse-transcriptases/reversetranscriptases_blog_thumb_1122.jpg?rev=73b3c96a0fb54cbbac83426504182b4a&sc_lang=en-us'/><p>Read about the difference between retroviral reverse transcriptases and group II intron-encoded reverse transcriptases and why one is a better choice for cDNA synthesis from long transcripts.</p> Wed, 16 Nov 2022 00:00:00 Z 2022-11-17T16:49:54Z ca295fb0-7538-4be3-b012-d48e6e323b6e https://www.neb.com/en-us/nebinspired-blog/getting-started-with-loop-mediated-isothermal-amplification Getting started with loop-mediated isothermal amplification (LAMP) <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/getting-started-with-loop-mediated-isothermal-amplification/lampvspcr_blog_thumb_1022.jpg?rev=e62f9809a714499986513cc2ca68d67a&sc_lang=en-us'/><p>Are you thinking about using loop-mediated isothermal amplification (LAMP) but still have questions? In this blog post, we answer some of the most frequently asked questions we get about LAMP.&nbsp;</p> Wed, 12 Oct 2022 00:00:00 Z 2022-10-19T20:05:34Z 7a25c674-2d7a-4547-95db-8266d5abf6ff https://www.neb.com/en-us/nebinspired-blog/four-tips-for-pcr-amplification-of-gc-rich-sequences Four tips for PCR amplification of GC-rich sequences <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/4-tips-for-gc-rich-pcr-amplification/gcrich_pcr_blog_thumb_0822.jpg?rev=ce86ab88b8724465a04d22ad7da7498a&sc_lang=en-us'/><p>GC-rich DNA sequences can be difficult to amplify. In this blog post, we've outlined four key considerations from NEB scientists to troubleshoot your GC-rich PCR.</p> Thu, 15 Sep 2022 00:00:00 Z 2022-10-06T17:20:01Z 4cc016a8-fd7d-45e6-9e14-cf0dd84e91ec https://www.neb.com/en-us/nebinspired-blog/the-right-stem-program-can-open-the-door-to-a-stem-career The right STEM program can open the door to a STEM career <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/the-right-stem-program-can-open-the-door-to-a-stem-career/steam_education_blog_thumb_0822.jpg?rev=04bf0866e7124741aff1d4b190c6fe6f&sc_lang=en-us'/><p>This blog will walk you through how to identify nearby programs that can introduce students to future careers in biology related fields.</p> Mon, 29 Aug 2022 00:00:00 Z 2023-03-02T16:18:42Z 13c196de-614a-41c9-90a6-b3f65ccd0c87 https://www.neb.com/en-us/nebinspired-blog/which-dna-ligase-is-the-best-choice-for-dna-assembly Which DNA ligase is the best choice for DNA assembly? <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/ligase-choice-has-a-strong-impact-on-dna-assembly/ligasechoice_blog_thumb_0722.jpg?rev=18704327941d47e5a568e599d0e4dd6f&sc_lang=en-us'/><p>NEB researcher Katie Bilotti Ph.D. shares how deep characterization of end joining reactions reveals that ligase choice is vital for optimizing DNA assembly reactions, and more.</p> Thu, 14 Jul 2022 00:00:00 Z 2023-08-31T14:41:38Z 3a8f02bd-eb2e-475a-bb01-de5625b3c37b https://www.neb.com/en-us/nebinspired-blog/monitoring-tick-and-mosquito-infections-using-lamp Monitoring tick and mosquito infections using LAMP: Screening for EEE and beyond <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/monitoring-tick-and-mosquito-infections-using-lamp/eee_lamp_blog_thumb_0522.jpg?rev=9268ed7fe6ea4d48902ddf1c0d8ccbea&sc_lang=en-us'/><p><em>The Carlow lab&rsquo;s research and development of new field-friendly diagnostics for infectious disease is empowered by the loop-mediated isothermal amplification (LAMP) assay. A recent collaboration shows LAMP&rsquo;s merits for monitoring mosquitoes for the EEE virus.</em></p> Wed, 22 Jun 2022 00:00:00 Z 2023-03-02T16:26:16Z 2248d8c8-fbbc-41f1-bb82-dc202e91de92 https://www.neb.com/en-us/nebinspired-blog/guiding-crispr-cas13-activity-using-chemically-modified-guides Guiding CRISPR/Cas13 Activity Using Chemically Modified Guides <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/guiding-crisprcas13-activity-using-chemically-modified-guides/crispr_cas13_blog_thumb_0522.jpg?rev=90a2abbda8e94bdf8f68eb82317c7f3e'/><p><p>Dr. Neville Sanjana, a core faculty member at the New York Genome Center, explains his recent collaboration and publication detailing the use of chemically modified guide RNAs to enhance CRISPR-Cas13 knockdown in human cells.</p></p> Thu, 02 Jun 2022 00:00:00 Z 2022-06-03T19:34:32Z a9674960-cd45-4aac-84f2-8598f7c64b05 https://www.neb.com/en-us/nebinspired-blog/merging-art-and-science-an-alternative-science-career Merging art &amp; science &ndash; an alternative science career <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/merging-art-and-science/scientificillustrator_career_blog_thumb_0422.jpg?rev=7ff9e520d21c4e6d9f8c50a5bcbbfb32'/><p>Tasha Jos&eacute;, NEB's Scientific Illustrator, describes her exciting job role combining two of her passions - science &amp; art.</p> Thu, 07 Apr 2022 00:00:00 Z 2022-09-15T16:17:53Z 0c58e203-35c1-42ad-b856-6d1fe090c062 https://www.neb.com/en-us/nebinspired-blog/bringing-the-international-space-station-into-the-molecular-age Bringing the International Space Station into the Molecular Age <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/bringing-the-international-space-station-into-the-molecular-age/iss_blog_thumb_0322.jpg?rev=e3e2b0e6370241e28ffdd6a99e51135c'/><p>With sights set on returning to the Moon and eventually Mars, NASA microbiologists create modified-for-spaceflight molecular workflows to diagnose potential medically significant pathogens in near real-time, entirely onboard the spacecraft.</p> Thu, 10 Mar 2022 00:00:00 Z 2023-09-09T12:21:59Z a050f6a1-4491-4d93-a15a-930edd1a114c https://www.neb.com/en-us/nebinspired-blog/bisulfite-sequencing-but-at-what-cost Bisulfite sequencing, but at what cost? <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/bisulfite-sequencing-but-at-what-cost/nebinspired_bisulfite_seq_blog_thumb_0122.jpg?rev=1cf51e75b6574b7ebb9d3f2d538f8ca9'/><p>Why do so many core sequencing labs decline to perform bisulfite-seq? Let&rsquo;s review the intrinsic risks of bisulfite conversion and then discuss how enzymatic cytosine conversion overcomes these challenges, enabling more biologically meaningful results at a lower cost with application versatility.</p> Thu, 17 Feb 2022 00:00:00 Z 2022-09-15T16:24:49Z 2595c466-21be-43e8-9d02-a244a2c03902 https://www.neb.com/en-us/nebinspired-blog/bringing-more-covid-19-testing-to-africa Bringing more COVID-19 testing to Africa <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/bringing-more-covid-19-testing-to-africa/covidtesting_africa_blog_thumb_0122.jpg?rev=8965d17154c5451182c92f8fde7bab51'/><p>With so few Africans vaccinated against COVID-19, simple and sensitive tests are critical for saving lives.&nbsp; NEB is donating reagents to aid in the development of diagnostic tests (cLAMP) that can be used instead of PCR and can deliver rapid results detected by a simple color change.&nbsp; They are ideally suited for resource-poor areas by not requiring expensive laboratory equipment.</p> Thu, 27 Jan 2022 00:00:00 Z 2022-09-15T16:27:34Z 0100607b-bc7c-42b0-9b6e-0fbb5d91c727 https://www.neb.com/en-us/nebinspired-blog/proven-tips-for-pcr-primer-design Proven tips for PCR primer design <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/proven-tips-for-pcr-primer-design/nebinspired_pcr_primers_blog_thumb_1021.jpg?rev=b3a10cfa05604178b613d09be3553762'/><p>PCR optimization and validation is a longtime passion at NEB. Avoid common PCR pitfalls and obtain the amplicon you desire with these primer design tips from our experts.</p> Mon, 08 Nov 2021 00:00:00 Z 2022-09-15T14:17:17Z 6b420bbf-bbe5-4ca8-9edf-6529fad68794 https://www.neb.com/en-us/nebinspired-blog/using-large-scale-rna-sequencing-to-probe-immune-response-including-autoimmune-disease-and-vaccination Using large-scale RNA sequencing to probe immune response, including autoimmune disease and vaccination <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/using-large-scale-rna-sequencing-to-probe-immune-response/immuneseq_blog_thumb_0921.jpg?rev=ce39dd927c874450ba10d4734f42ab06'/><p>Researchers are efficiently exploring immune responses with a new bulk RNA sequencing workflow that generates full-length reads of B or T cell receptor V(D)J and (C) region mRNA. The discoveries incorporating this unique view are shedding light on previously unanswerable questions surrounding immune response to infection and therapeutic intervention.<br /></p> Wed, 13 Oct 2021 00:00:00 Z 2022-09-15T14:07:01Z 52e309a4-837b-43f5-bb09-e6c68a45e930 https://www.neb.com/en-us/nebinspired-blog/8-tips-to-follow-when-choosing-a-restriction-enzyme-for-in-vitro-mrna-transcription-for-vaccine-production 8 tips to follow when choosing a restriction enzyme for <em>in vitro</em> mRNA vaccine production<br /> <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/restriction-enzyme-requirements-for-in-vitro-mrna-transcription-for-vaccine-production/re_in_mrnavaccine_blog_thumb_0921.jpg?rev=20b36d2049154814a71999a5dd66e873'/><p>mRNA vaccines have emerged as alternatives to conventional vaccine approaches because of the streamlined path from early discovery and development to a point-of-care therapeutic. In this post, read practical tips to consider when choosing a restriction enzyme for one of the crucial first steps of IVT vaccine development - linearizing the plasmid that contains the vaccine target sequence.</p> Fri, 24 Sep 2021 00:00:00 Z 2022-06-02T16:59:18Z 872a555d-b3c1-4571-a2d0-df4d720392c5 https://www.neb.com/en-us/nebinspired-blog/bioinformatics-alerts-for-viral-variant-impacts-on-molecular-tests Bioinformatics alerts for viral variant impacts on molecular tests <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/bioinformatics-alerts-for-viral-variant-impacts-on-molecular-tests/primermonitor_blog_thumb_0821.jpg?rev=16496458576f4aad9e0c8e18d05e1818'/><p>NEB bioinformatics scientist Brad Langhorst, Ph.D., discusses the impact of emerging variants on existing SARS-CoV-2 molecular tests, the key factor in test failures, who benefits from using NEB's new, Primer Monitor tool, and lessons from the pandemic for infectious disease outbreaks.</p> Wed, 11 Aug 2021 00:00:00 Z 2022-06-03T19:33:29Z a39d402e-0b99-4dfd-9027-b9b7f5ed17e2 https://www.neb.com/en-us/nebinspired-blog/5-tips-to-stand-out-on-linkedin-with-a-great-scientist-profile 5 Tips to Stand out on LinkedIn with a Great Scientist Profile <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/5-tips-to-stand-out-on-linkedin-with-a-great-scientist-profile/linkedin_blog_thumb_0721.jpg?rev=ef65d4a208f941999e0ae90363353825'/><p><p>Life science recruiters and hiring managers frequently use LinkedIn to find job candidates. We spoke to a LinkedIn expert to explore how to make your LinkedIn profile help you find the perfect job in science.&nbsp; &nbsp;</p></p> Wed, 30 Jun 2021 00:00:00 Z 2022-09-14T14:19:09Z 2357e3d9-df9d-4759-a9ab-66a934364794 https://www.neb.com/en-us/nebinspired-blog/rt-qpcr-strategies-for-molecular-diagnostics-and-oem RT-qPCR strategies for molecular diagnostics, OEM and customized solutions <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/scientist-interview/nebinspired_rtqpcr_mdx_thumb_0621.jpg?rev=b09c803d05d148dfae7c08cfc7070339'/><p><p>NEB Scientist Greg Patton, Ph.D., discusses how the molecular diagnostics field improves assay accuracy, robustness, and accessibility, for COVID RT-qPCR testing - including molecular technical advantages, OEM and customized reagent considerations to put upfront, and a tool to monitor for viral variant effects.</p></p> Thu, 24 Jun 2021 00:00:00 Z 2022-06-21T08:09:47Z 187dc48e-0a97-4b4f-b8a9-763060e86eaa https://www.neb.com/en-us/nebinspired-blog/supersizing-molecular-cloning-by-rewriting-the-rules-for-golden-gate-assembly Supersizing Molecular Cloning by Rewriting the Rules for Golden Gate Assembly <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/supersizing-gga/nebinspired_gga_design_thumb_0621.jpg?rev=32ecf429c5584df0ba46570587862295'/><p>Read about how NEB scientists have rewritten the rules for Golden Gate Assembly of large multi-fragment targets - using Data-optimized Assembly Design (DAD) and modified protocols they have assembled the entire T7 bacteriophage genome from 52 fragments</p> Fri, 11 Jun 2021 00:00:00 Z 2023-08-15T17:30:48Z de832837-d9d6-40ff-b556-dd66e8286b48 https://www.neb.com/en-us/nebinspired-blog/preventing-covid-19-outbreaks-through-college-campus-surveillance-testing Preventing COVID-19 Outbreaks through College Campus Surveillance Testing <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/surveillance-testing/nebinspired_covid_surveillancetesting_thumb_0521.jpg?rev=b035815a36354575bbf3adc964247448'/><p>COVID-19 pooled testing is an efficient way to monitor viral spread within a community, and the loop mediated isothermal amplification (LAMP) assay is a major asset for community monitoring because it's inexpensive, fast, and accurate.</p> Wed, 19 May 2021 00:00:00 Z 2022-09-15T16:16:08Z d40677dc-8613-4f41-a946-0f9c42b4b2c2 https://www.neb.com/en-us/nebinspired-blog/bioremediation-for-sustainable-clean-water Bioremediation for sustainable clean water<br /> <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/earthday21_blog_wastewater_thumb_0421.jpg?rev=4cce51f7f80046d8bcc28509290c1d28'/><p>Read about how bioremediation has the potential to sustainably and economically address the global scarcity of potable water supplies, and how NEB puts bioremediation into practice to process campus wastewater.<br /></p> Thu, 29 Apr 2021 00:00:00 Z 2022-06-20T15:29:22Z a99c46a9-3e94-421f-a876-10d389b83d64 https://www.neb.com/en-us/nebinspired-blog/recycling-styrofoam Recycling styrofoam <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/styrofoam/earthday21_blog_styrofoam_thumb_0421.jpg?rev=f6f7e76d2bf0474dbc29b542bce7b126'/><p><p>Learn what it takes to achieve the most eco-friendly disposal for EPS foam coolers and about some of the sustainable shipping solutions used by NEB to deliver molecular biology tools to scientists around the world.</p></p> Wed, 21 Apr 2021 00:00:00 Z 2022-06-03T19:36:55Z a923c9ea-904f-4af1-a42e-6b00cc3d0eea https://www.neb.com/en-us/nebinspired-blog/marine-biodiversity Marine Biodiversity <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/what-percentage-of-species-on-earth-lives-below-the-surface-of-the-ocean/earthday21_blog_biodiversity_thumb_0421.jpg?rev=f3617d3bc7a147a4becf6ae7cac14276'/><p>Have you ever wondered what proportion of all of the species on Earth live in the ocean? Learn about marine biodiversity, what threatens it, and the actions NEB takes to help protect it for future generations.</p> Thu, 15 Apr 2021 00:00:00 Z 2023-03-16T18:38:51Z a4fc4ac3-49cf-4f27-b791-61757b4beb5e https://www.neb.com/en-us/nebinspired-blog/b-corp-certification-measures-environmental-sustainability-and-much-more B Corp&trade; Certification Measures Environmental Sustainability and Much More <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/b-corp-certification-measures-environmental-sustainability-and-much-more/earthday21_blog_b_corp_thumb_0421.jpg?rev=13a555ccc5e342b289f496cac6be2de3'/><p>Can a biotech be a B Corp&trade;? Yes! Learn about eco-friendly actions taken by New England Biolabs that helped the company earn B Corp certification.</p> Thu, 08 Apr 2021 00:00:00 Z 2023-03-02T17:33:45Z d161fa5a-c068-42d5-9d77-bda6d2bcda51 https://www.neb.com/en-us/nebinspired-blog/using-paqci-for-golden-gate-assembly Using PaqCI<sup>&reg;</sup> for Golden Gate Assembly - What Makes it a Special Addition to the NEB Assembly Portfolio? <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/using-paqci-for-golden-gate-assembly/paqci_blog_thumb_0321.jpg?rev=240591cd192242d29dadf1af78a7a0d5'/><p>Read about our new 7-base recognition sequence Type IIS restriction enzyme and how it can be used for simple to complex 24-fragment Golden Gate Assembly, achieving our highest level of efficiency and fidelity yet.</p> Wed, 07 Apr 2021 00:00:00 Z 2022-06-03T19:38:39Z f1d1b7e8-12e0-4321-a789-e379f91f5fec https://www.neb.com/en-us/nebinspired-blog/ten-ways-to-reduce-laboratory-waste Ten ways to reduce laboratory waste <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/lab-waste/earthday21_blog_lab_waste_thumb_0421.jpg?rev=230566dfb91946979d81f75d70a210c6'/><p>The best way to reduce laboratory waste is with "upstream thinking". Stop it before it starts. Here are ten actions that will help you to conserve life science lab resources and practice environmental stewardship, Earth Day and every day!</p> Thu, 01 Apr 2021 00:00:00 Z 2022-06-03T19:38:00Z 516d3f09-f5b3-423d-9e9a-fe81868658ed https://www.neb.com/en-us/nebinspired-blog/think-differently-about-your-molecular-diagnostics-partners Think differently about your molecular diagnostics partners <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/think-differently-about-your-molecular-diagnostics-supply-chain/mdx_blog_thumb_0321.jpg?rev=81df9164629d4eb7a4b1f255868ea78e'/><p>New England Biolabs&reg; (NEB&reg;) partners with customers globally to address the challenges faced by innovators developing the molecular diagnostics (MDx) technologies required to address public health and pandemic preparedness.<br /></p> Mon, 15 Mar 2021 00:00:00 Z 2022-06-03T19:38:12Z beae4584-fb0a-4a16-94be-4e51e148b1cb https://www.neb.com/en-us/nebinspired-blog/dr-peter-hotez-champions-low-cost-covid-19-vaccines-and-training-scientists-for-science-communication Dr. Peter Hotez champions low cost COVID-19 vaccines and training scientists for science communication <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/dr-peter-hotez/hotez_passioninscience_blog_thumb_0221.jpg?rev=1fdd8eec20c84ab1bae5921907a8b735'/><p>NEB Passion in Science Award&reg; Winner and vaccine researcher Dr. Peter Hotez shares how low-cost vaccine biotechnology is being used to expand COVID-19 vaccine production globally, the urgency of opposing anti-science rhetoric, and insights from his new book Preventing the Next Pandemic: Vaccine Diplomacy in a Time of Anti-science.</p> Mon, 01 Mar 2021 00:00:00 Z 2023-03-02T17:43:06Z b6acd4f9-31ed-4b9f-b94e-b73067dfdf3d https://www.neb.com/en-us/nebinspired-blog/hmw-dna---minimizing-the-bottleneck High Molecular Weight (HMW) DNA Extraction &ndash; minimizing a bottleneck for emerging long read sequencing applications <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/high-molecular-weight--dna-extraction-_-minimizing-a-bottleneck-for-emerging-long-read-sequencing-applications/monarchhmw_blog_thumb_0221.jpg?rev=1af65952df9b42759d1bd5eb673972b5'/><p>Extracting high quality, high molecular weight (HMW) DNA can be so time-consuming that it causes a bottleneck for the emerging, exciting downstream applications that require long DNA molecules as an input. Read about how scientists are addressing this bottleneck and how they are using the long read data to reveal a wealth of genomic information.</p> Tue, 16 Feb 2021 00:00:00 Z 2022-06-03T19:34:54Z f88c5a2b-026e-4c37-ad24-ce394807d08f https://www.neb.com/en-us/nebinspired-blog/harvesting-improved-methylome-sequencing-accuracy Harvesting improved methylome sequencing accuracy <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/harvesting-more-accurate-methylomes/nebinspired_methylomesequencing_1200x1200_1220/methylomesequencing_blog_thumb_1220.jpg?rev=60e746611958497684660910fb631a10'/><p><p>Plant epigenetics research is an attractive approach to enhance crops, rooted to accurate methylome analysis in a complex context. The performance of the Enzymatic Methyl-seq method merits replacing bisulfite conversion for high resolution plant methylation studies. Recently a team from the University of California Los Angeles took this leading-edge epigenomics method to task, comparing it to Whole Genome Bisulfite Sequencing (WGBS) results with <em>Arabidopsis thaliana</em> samples. Discover how researchers can improve studies of any eukaryotic methylome and you might become an EM-seq convert.</p></p> Mon, 14 Dec 2020 00:00:00 Z 2022-06-03T19:34:45Z d080fc35-7293-481f-932d-ed51787c9e77 https://www.neb.com/en-us/nebinspired-blog/research-lab-safety-during-covid-19 Research lab safety during COVID-19 <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/research-lab-safety-during-covid19/nebinspired_rebootbench_blog_thumb_1120.png?rev=fcaf4ab6029546e5903145226a3d30a8'/><p>Adjusting to SARS-CoV-2 working conditions in research labs takes some getting used to. Find help tips and printable signage to remind lab members to practice COVID-19 safety guidelines in this blog post.</p> Thu, 19 Nov 2020 00:00:00 Z 2022-06-03T19:37:15Z 971e4fd0-1a76-47e8-a906-b5c549a0e7d5 https://www.neb.com/en-us/nebinspired-blog/bacterial-art---incubating-creativity-in-the-lab Bacterial art - incubating creativity in the lab <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/bacterial-art---incubating-creativity-in-the-lab/bacterialart_blog_thumb_0520.jpg?rev=4325b845b9d746da8c98487f93593475'/><p>Read about how science and art have become increasingly compartmentalized over time due to a focus on specialization. As a result, scientists communicate with scientists, and artists communicate with artists. Yet, there are parallels in the creative process and spirit behind both the scientific and artistic endeavors, and they are both studies and interpretations of the world around us. Using bacterial art, one lab at New England Biolabs encourages a mutualistic relationship between science and art, allowing the two cultures to inform and enrich each other.</p> Wed, 21 Oct 2020 00:00:00 Z 2022-06-16T15:45:26Z 56965e63-d89a-4c9a-b97e-edb52ab254c9 https://www.neb.com/en-us/nebinspired-blog/sars-cov-2-rt-qpcr-workflow-research-to-advance-clinical-testing SARS-CoV-2 RT-qPCR workflow research to advance clinical testing <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/sars-cov-2-rt-qpcr-research-simplifying-rt-qpcr-workflows-to-increase-covid-19-testing-accessibility/nebinspired_rnaextraction_thumb_0920.jpg?rev=8f49523f1b0146a28ad02ba26f9845aa'/><p>Creating streamlined workflows for COVID-19 testing remains a key step in solving the public health crisis. What sort of features will help make these tests more accessible and useful for clinicians? Let's consider that question with a look at the challenges set by the NIH RADx Initiative, the value of qPCR-based SARS-CoV-2 tests, new options from New England Biolabs for saliva samples and plug and play automation, and the recent call for a new testing paradigm.</p> Mon, 05 Oct 2020 00:00:00 Z 2022-06-21T08:13:03Z 87eddaf8-51a9-4fc0-892b-212fb6fe4d9c https://www.neb.com/en-us/nebinspired-blog/loop-mediated-isothermal-amplification-allows-testing-to-be-performed-anywhere Loop-mediated isothermal amplification allows testing to be performed anywhere <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/lamp_blog_thumb_0520.jpg?rev=295afc01129a4be3ba2f9b41cc69989c'/><p>Loop-mediated isothermal amplification (LAMP) is a fast-growing technology with a wide range of applications in human health and medical diagnosis. Because LAMP does not require the specialized equipment typically found in a molecular laboratory, it can simply and inexpensively be applied in a much broader range of settings. Read about how LAMP is used in COVID-19 research, vector-borne illness control programs, agriculture, and even on the International Space Station.</p> Tue, 08 Sep 2020 04:00:00 Z 2023-03-16T20:01:35Z 6a142c5e-199e-4bb8-9987-ac29d66d260c https://www.neb.com/en-us/nebinspired-blog/reforestation-offers-benefits-over-carbon-credits Reforestation Offers Benefits Over Carbon Credits <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/reforestation_blog_thumb_0520.jpg?rev=9ea0dfea6947495e926bd7e933345297'/><p>Could reforestation efforts be the key to controlling climate change? Trees are able to sequester carbon efficiently, but they offer more than that. Trees also help control ground temperature, extend animal habitats and provide an income source to farmers. Reforestation offers broader reaching benefits than other carbon emission offset measures.</p> Tue, 08 Sep 2020 04:00:00 Z 2022-06-20T15:39:51Z 9266d761-4d3c-40a3-874f-65d63d2ccf3b https://www.neb.com/en-us/nebinspired-blog/large-scale-mrna-synthesis-streamlined-thermostable-rna-polymerase Large scale mRNA synthesis could be streamlined with thermostable RNA polymerase research advance <img src='https://www.neb.com/en-us/-/media/nebus/page-images/blogs/blog-posts/rna_blog_thumb_0520.jpg?rev=bbc7c719776945d2895103b531a83366'/><p>Once a successful mRNA based vaccine is identified for COVID-19, the next hurdle for biopharma is producing a world supply of therapeutic mRNA. Production efficiency will be paramount for this undertaking! As RNA synthesis is evolving for use in medicine, RNA polymerase research is key to improving how RNA is made. Learn about an advance that could streamline conventional mRNA manufacturing workflows by safely circumventing the need for costly purification in this interview with NEB RNA Research Staff Scientist, Bijoyita Roy, Ph.D.</p> Tue, 08 Sep 2020 04:00:00 Z 2022-06-20T15:35:05Z