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Product Information
NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.
The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB #M0203) which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.
The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).- This product is related to the following categories:
- DNA Ligases Products
- This product can be used in the following applications:
- Blunting,
- Phosphorylation (Kinase),
- Cloning Ligation, Fast Cloning: Accelerate your cloning workflows with reagents from NEB
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