LongAmp^® Taq PCR Kit

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E5200S     -20       LongAmp® Taq DNA Polymerase M0323SVIAL -20 1 x 0.2 ml 2,500 units/ml   Deoxynucleotide (dNTP) Solution Mix N0447SVIAL -20 1 x 0.2 ml 10 mM each dNTP   LongAmp® Taq Reaction Buffer Pack B0323SVIAL -20 1 x 1.5 ml 5 X   Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM   Nuclease-free Water B1502AVIAL -20 2 x 1.5 ml Not Applicable

Application Features

• Long Range PCR
• Colony PCR

Companion Products

• Deoxynucleotide (dNTP) Solution Set
• LongAmp® Taq DNA Polymerase
• Magnesium Sulfate (MgSO₄) Solution
• LongAmp® Hot Start Taq 2X Master Mix
• LongAmp® Hot Start Taq DNA Polymerase

1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. 91, 2216-2220.
2. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
5. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

1. Protocol for LongAmp™ Taq PCR Kit

• Activity of Restriction Enzymes in PCR Buffers
• Guidelines for PCR Optimization with Taq DNA Polymerase
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• DNA Polymerase Selection Chart
• Thermophilic DNA Polymerases

• PCR Selector
• Tm Calculator

1. What is the recommended enzyme amount when using LongAmp™ Taq DNA Polymerase?
2. Can the extension step be carried out at 72°C when using LongAmp™ Taq DNA Polymerase?
3. What is the extension rate when using LongAmp™ Taq DNA Polymerase?
4. Why is the product a smear when visualized on an agarose gel?
5. Can the LongAmp™ kit be used to amplify GC-rich amplicons?
6. What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Taq DNA Polymerase?
7. What is the fidelity of the LongAmp™ Taq DNA Polymerase compared to Taq DNA Polymerase?
8. Can NEBuilder^® HiFi DNA Assembly master mix remove both 3′ and 5′ end mismatches? 

• PCR Troubleshooting Guide
• Taq PCR Kit Troubleshooting Guide

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E5200S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E5200S_v1_0411712
• E5200S_v1_10013129
• E5200S_v1_10015908
• E5200S_v1_10024730
• E5200S_v1_10029415
• E5200S_v1_10034811
• E5200S_v1_10043958
• E5200S_v1_10052221
• E5200S_v1_10061500
• E5200S_v1_10067259
• E5200S_v1_10069146
• E5200S_v1_10073017
• E5200S_v1_10080294
• E5200S_v1_10088161
• E5200S_v1_10092965
• E5200S_v1_10100863
• E5200S_v1_10108304
• E5200S_v1_10112948
• E5200S_v1_10119049
• E5200S_v1_10135893
• E5200S_v1_10147001
• E5200S_v1_10159710
• E5200S_v1_10164994
• E5200S_v1_10177199
• E5200S_v1_10180118
• E5200S_v1_10184727
• E5200S_v1_10197193
• E5200S_v1_10208708
• E5200S_v1_10219593
• E5200S_v1_10234526

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• LongAmp^® Taq DNA Polymerase
• Deoxynucleotide (dNTP) Solution Mix
• LongAmp^® Taq Reaction Buffer Pack
• Magnesium Sulfate (MgSO₄) Solution
• Nuclease-free Water

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Purchase of this product provides the purchaser with a non-exclusive license to use LongAmp® Taq PCR Kit for research purposes only. Commercial use of this product may require a license from New England Biolabs, Inc. under U.S. Patent No. 5,352,778.

Trademarks

LONGAMP® and NEW ENGLAND BIOLABS^® are registered trademarks of New England Biolabs, Inc.
DEEP VENT® is a trademark of New England Biolabs, Inc.

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