New England Biolabs has called upon its 40 years of expertise in enzymology to develop several solutions for epigenetics research, including NEBNext® Enzymatic Methyl-seq (EM-seq™) and the EpiMark® suite of products.
A new method for DNA methylation analysis at the single base level, NEBNext Enzymatic Methyl-seq (NEB #E7120), is now available. This enzyme-based technology minimizes damage to DNA and produces high quality libraries that enable superior detection of 5mC and 5hmC from fewer sequencing reads.
Easy-to-use EpiMark kits simplify DNA methylation (5mC) and hydroxymethylation (5hmC) detection and analysis, as well as ChIP, histone and nucleosome analysis. Independently applicable, individual epigenetics reagents also complement the EpiMark® kits. NEB's methylation- and hydroxymethylation- sensitive or dependent enzymes, DNA methyltransferases and DNA controls are all useful for mapping DNA modifications and methylating DNA at specific sites for gene expression studies. Our protein methyltransferases and recombinant histones perform efficiently in protein modification and characterization studies. Our range of modified and unmodified genomic DNAs can be used as controls for detection of DNA methylation. Our series of human DNA (cytosine-5) methyltransferase (DNMT) antibodies are ideally suited for Western blots and immunoprecipitation.
Our complete Epigenetics suite is expertly designed for optimized research and discovery.
EpiMark® is a registered trademark of New England Biolabs, Inc.
EM-seq™ is a trademark of New England Biolabs, Inc.
Epigenetics - Expanding on Genomic Foundations
- Epigenetics Brochure
- EpiMark® Methylated DNA Enrichment Kit Troubleshooting Guide
- Small RNA-Mediated DNA Methyltransferase 1 Inhibition Causes DNA Methylation Alteration in Mammalian Cells (2015)
- NicE-seq: High Resolution Open Chromatin Profiling (2018)
- EM-seq™ Enables Accurate and Precise Methylome Analysis of Challenging DNA Samples (2019)
- Enzymatic Methyl-seq: Next Generation Methylomes (2019)
- Uncovering the Cannabis sativa Methylome Through Enzymatic Methyl-seq (2019)
- EM-seq™ enables accurate and robust methylation detection of cell free DNA and FFPE DNA sample types
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If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.