mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA.
mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure.
Recently, it has been reported that the 2′O methylation in the cap 1 structure helps the mRNA evade innate immune response in some cell types in vivo (1,2).
mRNA Cap 2´-O-Methyltransferase specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5´ end (3,4). Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme (NEB #M2080 ).
The reagents provided in this pack can be used to methylate up to 400 μg of capped RNA.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Capping Buffer||10 X|
|S-adenosylmethionine (SAM)||-20||32 mM|
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