Q5® High-Fidelity DNA Polymerase

Home PCR, qPCR & Amplification Technologies Products Q5^® High-Fidelity DNA Polymerase

Catalog #	Concentration	Size	List Price	Your Price
M0491S	2,000 units/ml	100 units	$128.00	Sign In Sign In
M0491L	2,000 units/ml	500 units	$508.00	Sign In Sign In

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Product Information
Q5^® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg⁺⁺ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.

Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity DNA Polymerase. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip^® analysis.

Product Source
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.

This product is related to the following categories:

Q5® High-Fidelity DNA Polymerases Products

This product can be used in the following applications:

Gibson Assembly®,

Long Range PCR,

Fast PCR,

High-Fidelity PCR,

Multiplex PCR,

Specialty PCR,

Routine PCR,

DNA Amplification, PCR & qPCR,

PCR,
Fast Cloning: Accelerate your cloning workflows with reagents from NEB
- Properties & Usage
  
  Unit Definition
  One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.
  Unit Assay Conditions
  25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl₂, 1 mM β-mercaptoethanol, 200 μM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.
- Protocols, Manuals & Usage
  - Protocols
    
    PCR Using Q5® High-Fidelity DNA Polymerase (M0491)
  - Usage Guidelines
    
    Activity of Restriction Enzymes in PCR Buffers
    
    Guidelines for PCR Optimization with Thermophilic DNA Polymerases
  - Application Notes
    
    AppNote_Multiplex_PCR_Using_Q5_HF_DNA_Polymerase
- Tools & Resources
  - Selection Charts
    
    DNA Polymerase Selection Chart
  - Web Tools
    
    PCR Selector
    
    Tm Calculator
- FAQs & Troubleshooting
  - FAQs
    
    What are the advantages of using Q5^® High-Fidelity DNA Polymerase?
    
    What are the differences between the numerous Q5^® Polymerase products available?
    
    My results are not as expected. Where can I find troubleshooting help?
    
    What ends will my PCR products have?
    
    What does exonuclease activity mean for a DNA polymerase?
    
    Does Q5^® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
    
    What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
    
    How should I determine the appropriate annealing temperature for my reaction?
    
    What should the final primer concentration be in my PCR?
    
    When and how should I use the Q5^® High GC Enhancer?
    
    Do other polymerases work in Q5^® Reaction Buffer?
    
    There is a precipitate in the bottom of the buffer tube. Is this normal?
  - Troubleshooting
    
    PCR Troubleshooting Guide
- Citations & Technical Literature
  - Citations
    
    Product Citation Tool
    
    Powered by Bioz See more details on Bioz
    
    Additional Citations
    
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    Yonghe Zhang, Huiming Huang, Shanshan Xu, Bo Wang, Jianhua Ju, Huarong Tan, Wenli Li (2015) Activation and enhancement of Fredericamycin A production in deepsea-derived Streptomyces somaliensis SCSIO ZH66 by using ribosome engineering and response surface methodology. Microb Cell Fact; 14, 64. PubMedID: 25927229, DOI: 10.1186/s12934-015-0244-2
    
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    Longhai Dai, Can Liu, Yueming Zhu, Jiangsheng Zhang, Yan Men, Zeng Yan, Yuanxia Sun (2015) Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii. Plant Cell Physiol; , PubMedID: 25759326, DOI: 10.1093/pcp/pcv043
    
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    Yuan Xue, Jossef Osborn, Anand Panchal, Jay L Mellies (2015) The RpoE Stress Response Pathway Mediates Reduction of the Virulence of Enteropathogenic Escherichia coli by Zinc. Appl Environ Microbiol; 81, 3766-74. PubMedID: 25819956, DOI: 10.1128/AEM.00507-15
    
    Christine Henke, Pamela L Strissel, Maria-Theresa Schubert, Megan Mitchell, Claus C Stolt, Florian Faschingbauer, Matthias W Beckmann, Reiner Strick (2015) Selective expression of sense and antisense transcripts of the sushi-ichi-related retrotransposon - derived family during mouse placentogenesis. Retrovirology; 12, 9. PubMedID: 25888968, DOI: 10.1186/s12977-015-0138-8
    
    Binyamin D Berkovits, Christine Mayr (2015) Alternative 3' UTRs act as scaffolds to regulate membrane protein localization. Nature; , PubMedID: 25896326, DOI: 10.1038/nature14321
    
    Wang XJ, Zhang XJ, Hu W, Zhang TY, Wang SQ (2014) A simple and efficient strategy for the de novo construction of greater-than-genome-length hepatitis B virus replicons J Virol Methods; 207, 158-62. PubMedID: 25025817, DOI: 10.1016/j.jviromet.2014.07.009
    
    Vidhyadhar Nandana, Sushant Singh, Abhay Narayan Singh, Vikash Kumar Dubey (2014) Procerain B, a cysteine protease from Calotropis procera, requires N-terminus pro-region for activity: cDNA cloning and expression with pro-sequence. Protein Expr Purif; 103C, 16-22. PubMedID: 25173974, DOI: 10.1016/j.pep.2014.08.003
    
    Martin Kostovcik, Craig C Bateman, Miroslav Kolarik, Lukasz L Stelinski, Bjarte H Jordal, Jiri Hulcr (2014) The ambrosia symbiosis is specific in some species and promiscuous in others: evidence from community pyrosequencing. ISME J; , PubMedID: 25083930, DOI: 10.1038/ismej.2014.115
    
    Bert De Rybel, Milad Adibi, Alice S. Breda, Jos R. Wendrich, Margot E. Smit, Ondej Novk, Nobutoshi Yamaguchi, Saiko Yoshida, Gert Van Isterdael, Joakim Palovaara, Bart Nijsse, Mark V. Boekschoten, Guido Hooiveld, Tom Beeckman, Doris Wagner, Karin Ljung, Christian Fleck, Dolf Weijers (2014) Integration of growth and patterning during vascular tissue formation in Arabidopsis Science; 345, 1255215. PubMedID: 25104393, DOI: 10.1126/science.1255215
    
    Xin Duan, Arjun Krishnaswamy, Irina De la Huerta, Joshua R Sanes (2014) Type II Cadherins Guide Assembly of a Direction-Selective Retinal Circuit. Cell; 158, 793-807. PubMedID: 25126785, DOI: 10.1016/j.cell.2014.06.047
    
    Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013) ROLE OF HUMAN HYPOXANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR) Mol Pharmacol; 87247. PubMedID: 23907213
    
    Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc (2013) Hedgehog Signaling Regulates MyoD Expression and Activity J Biol Chem; 288(6), 4389–4404. PubMedID: 23266826
    
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  - Feature Articles
    
    Polymerase Fidelity: What is it, and what does it mean for your PCR?