PCR, qPCR & Amplification Technologies
Choose Type:
- Q5® High-Fidelity DNA Polymerases
- OneTaq® DNA Polymerases
- Taq DNA Polymerases
- LongAmp® DNA Polymerases
- Luna® qPCR & RT-qPCR Products
- Other PCR Polymerases
- Phusion® High-Fidelity DNA Polymerases
- Master Mixes
- DNA Manipulation
- Isothermal Amplification & Strand Displacement
- Nucleotide Solutions
- cDNA Synthesis & Reverse Transcriptases
- RT-PCR
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Understanding Variability in DNA Amplification Reactions
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DNA Damage - the major cause of missing pieces from the DNA puzzle
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Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
- Isothermal Amplification Trifold
- Luna Universal qPCR And RT-qPCR
- PCR Brochure
- NEB LAMP Primer Design Tool
- PCR Selector
- DNA Polymerase Selection Chart
- Luna® One-Step RT-qPCR Troubleshooting Guide
- Luna® qPCR Troubleshooting Guide
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
- Choosing RNA Input Amounts for NEB T2010
- DNA Damage and PreCR
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
- Optimization Tips for Luna® One-Step RT-qPCR
- Optimization Tips for Luna® qPCR
- Molecular Diagnostics for Gastrointestinal Parasites and Impact on Intestinal Microbiota in Rural Agentinian Children (2015)
- Amplification Reagents for Molecular Diagnostics Applications (2017)
- Colorimetric LAMP: Visual Detection for Simple Diagnostics (2017)
- A Single-tube, Low Input Protocol for Long Read Sequencing (2019)
Feature Articles
Brochures
Web Tools
Selection Tools
Troubleshooting Guides
Usage Guidelines
Posters
- Vladimir Potapov, Jennifer L. Ong. 2017. Examining Sources of Error in PCR by Single-Molecule Sequencing. PLOS One. , PubMedID: 28683110, DOI:
- Gardner, A.F., Jackson, K.M., Boyle, M.M., Buss, J.A., Potapov, V., Gehring, A.M., Zatopek, K.M., Correa, I.R., Jr., Ong, J.L., Jack. W.E. 2019. Therminator DNA polymerase: Modified nucleotides and unnatural substrates Front Mol Biosci. 6, PubMedID: 31069234, DOI: 10.3389/fmolb.2019.00028
- Poole, C.B., Sinha, A., Ettwiller, L., Apone, L., McKay, K., Panchapakesa, V., Lima, N.F., Ferreira, M.U., Wanji, S., Carlow, C.K.S 2019. <em>In silico</em> identification of novel biomarkers and development of new rapid diagnostic tests for the filarial parasites Mansonella perstans and Mansonella ozzardi Sci Rep. 9 (1), PubMedID: 31311985, DOI: 10.1038/s41598-019-46550-9
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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Overview of PCR
This overview will walk you through how the Polymerase Chain Reaction (PCR) works.
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Overview of qPCR
Learn the basics of qPCR in this short animation.
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“Dots in Boxes" Visualization of qPCR Data
Learn how we analyzed plates and plates of qPCR data, and how Luna stacks up to the competition.
