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IMPACT System

The IMPACT™ (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements, termed inteins, to separate the target protein from the affinity tag.

  • Distinguishes itself from all other purification systems by its ability to purify, in a single chromatographic step, a native recombinant protein without the use of a protease
  • Able to produce a target protein without vector–derived amino acids
  • Fusion to either C-terminus or N-terminus of target protein
  • Isolation of proteins with or without an N-terminal methionine residue
  • Ligation and labeling of recombinant proteins
  • T7 promoter-driven system to achieve high levels of expression and tight transcriptional control in E. coli

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FAQs for IMPACT System
Protocols for IMPACT System
Application Notes for IMPACT System
Schematic illustration of the IMPACT System
Figure 2: Schematic of the IMPACT System


Flow chart for Protein Expression and Purification using the IMPACT System
Figure 5: Flow chart for Protein Expression and Purification using the IMPACT System
Sample collection for analysis by SDS-PAGE is indicated.
Intein-mediated Protein Ligation (IPL)
Figure 3: Intein-mediated Protein Ligation (IPL)


Polylinkers in the vectors pTXB1 and pTYB21
Figure 4: Polylinkers in the vectors pTXB1 and pTYB21 
▼ indicates intein cleavage site.
Purification of Maltose Binding Protein (MBP) in a single affinity purification step
Figure 1: Purification of Maltose Binding Protein (MBP) in a single affinity purification step
Lane 1: uninduced cell extract. Lane 2: induced cell extract showing expressed fusion protein. Lane 3: MBP fractions eluted after inducing cleavage overnight at 4°C. Lane 4: MBP ligated to a peptide containing an N-terminal cysteine. Marker M is the Protein Ladder (NEB #P7703).
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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