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Product Information
NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
Restriction Enzyme Competitor Study: Nuclease Contamination
EcoRI, NotI, and BamHI from multiple suppliers were tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3’overhang or 5’ overhang containing oligonucleotides. The percent degradation is determined by capillary electrophoresis and peak analysis. The resolution is at the single nucleotide level.Product Source
An E. coli strain that carries the cloned and modified NruI gene from Nocardia rubra (ATCC 15906).- This product is related to the following categories:
- Restriction Endonucleases: N-O Products,
- High-Fidelity (HF®) Restriction Endonucleases Products
- This product can be used in the following applications:
- Restriction Enzyme Digestion
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Protocols, Manuals & Usage
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Product Citation Tool
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Featured Videos
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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NEB® TV Ep. 15 – Applications of Restriction Enzymes
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Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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Why is My Restriction Enzyme Not Cutting DNA?
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Restriction Enzyme Digest Problem: Too Many DNA Bands
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Double Digestion with NEBcloner
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