A lot of our customers want to know if it’s OK if foam is created during mixing the NEBNext reagents. A little bit of foam is actually OK because it’s important that the samples are mixed well.
To avoid excessive foam, you can set your pipette to 10-20% below the total well volume and you can keep your pipette tip in the sample at all times. During the last mix, be sure to dispense very slowly so all the sample can come out of the tip. If you still think you have a lot of foam you can do a brief centrifugation step to collapse all of the bubbles.
If you have any further questions you can always reach us at info@neb.com.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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